期刊
JOURNAL OF CLINICAL PERIODONTOLOGY
卷 42, 期 4, 页码 380-389出版社
WILEY-BLACKWELL
DOI: 10.1111/jcpe.12379
关键词
cell differentiation; invivo; mesenchymal stem cells; periodontal regeneration; tissue engineering
资金
- Royal Netherlands Academy of Arts and Sciences (KNAW) [CEP-10CDP025]
- National Natural Science Foundation of China [81371170]
- Ministry of Education of China [20120141130009]
AimThe implantation of bone marrow-derived mesenchymal stem cells (MSCs) has previously been shown successful to achieve periodontal regeneration. However, the preferred pre-implantation differentiation strategy (e.g. maintenance of stemness, osteogenic or chondrogenic induction) to obtain optimal periodontal regeneration is still unknown. This invivo study explored which differentiation approach is most suitable for periodontal regeneration. Materials and MethodsMesenchymal stem cells were obtained from Fischer rats and seeded onto poly(lactic-co-glycolic acid)/poly(-caprolactone) electrospun scaffolds, and then pre-cultured under different invitro conditions: (i) retention of multilineage differentiation potential; (ii) osteogenic differentiation approach; and (iii) chondrogenic differentiation approach. Subsequently, the cell-scaffold constructs were implanted into experimental periodontal defects of Fischer rats, with empty scaffolds as controls. After 6weeks of implantation, histomorphometrical analyses were applied to evaluate the regenerated periodontal tissues. ResultsThe chondrogenic differentiation approach showed regeneration of alveolar bone and ligament tissues. The retention of multilineage differentiation potential supported only ligament regeneration, while the osteogenic differentiation approach boosted alveolar bone regeneration. ConclusionChondrogenic differentiation of MSCs before implantation is a useful strategy for regeneration of alveolar bone and periodontal ligament, in the currently used rat model.
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