期刊
BONE
卷 42, 期 6, 页码 1226-1234出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2008.02.007
关键词
osteogenesis; silk scaffold; hydroxyapatite; tissue engineering; stem cells
资金
- NIBIB NIH HHS [P41 EB002520-05, R01 EB003210-06, P41 EB002520, R01 EB003210, R01 EB003210-04] Funding Source: Medline
- NIDCR NIH HHS [R01 DE013405-04, R01 DE013405] Funding Source: Medline
Silk fibroin biomaterials are being explored as novel protein-based systems for cell and tissue culture. In the present study, biomimetic growth of calcium phosphate on porous silk fibroin polymeric scaffolds was explored to generate organic/inorganic composites as scaffolds for bone tissue engineering. Aqueous-derived silk fibroin scaffolds were prepared with the addition of polyaspartic acid during processing, followed by the controlled deposition of calcium phosphate by exposure to CaCl2 and Na2HPO4. These mineralized protein-composite scaffolds were subsequently seeded with human bone marrow stem cells (hMSC) and cultured in vitro for 6 weeks under osteogenic conditions with or without BMP-2. The extent of osteoconductivity was assessed by cell numbers, alkaline phosphatase and calcium deposition, along with immunohistochemistry for bone-related outcomes. The results suggest increased osteoconductive outcomes with an increase in initial content of apatite and BMP-2 in the silk fibroin porous scaffolds. The premineralization of these highly porous silk fibroin protein scaffolds provided enhanced outcomes for the bone tissue engineering. (C) 2008 Elsevier Inc. All rights reserved.
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