期刊
PLANT SIGNALING & BEHAVIOR
卷 7, 期 12, 页码 1648-1652出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/psb.22294
关键词
aquaporin; plasma membrane intrinsic protein; PIP; HvPIP2;8; water transport; barley; Hordeum vulgare; Xenopus laevis oocytes
Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future.
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