期刊
BMC PLANT BIOLOGY
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2229-14-133
关键词
Abscisic acid (ABA) signaling; Abiotic stresses; Calcium-dependent protein kinase (CDPK); Drought stress tolerance; Rice; Spikelet fertility
资金
- Key Project of S & T Research of MoE of China [109105]
- Research Fund for the Doctoral Program of Higher Education of China [2012014211075]
- Open Research Fund of State Key Laboratory of Hybrid Rice in Wuhan University [KF201302]
- Major Technology Project of Hainan [ZDZX2013023-1]
Background: In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility. Results: We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes. Conclusions: Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity.
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