期刊
BMC PLANT BIOLOGY
卷 10, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2229-10-33
关键词
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资金
- PIP-CONICET [5145]
- UBACyT [X155]
- BID-OC-AR 1728 [PICT2005, 31656, PICT2007, 01976]
- USDA Current Research Information System [5335-21000-030-00D]
Background: LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts. Results: Here we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single similar to 3,550 Da compound ( as determined by UV-MALDI-TOF MS) that we named STIL ( for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner. Conclusion: We propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth.
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