期刊
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
卷 1, 期 1, 页码 39-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bcab.2011.08.009
关键词
Thermomyces lanuginosus; Immobilization; Olive pomace; Optimization; Response surface methodology
资金
- Mustafa Kemal University Research Foundation [1001M0114]
In this work, microbial lipase from Thermomyces lanuginosus was immobilized onto olive pomace. Response surface methodology was used to optimize the conditions for the maximum activity and to understand the significance and interaction of the factors affecting the specific activity. 5-level-3-factor central composite design was employed to evaluate the effects of immobilization parameters such as enzyme concentration (3-15%, v/v), pH (5.0-9.0) and buffer concentration (20-100 mM) on the specific activity of immobilized lipase. Quadratic polynomial equation was obtained for predicting specific activity of immobilized lipase. The predicted specific activity was 6.00 mu mol p-NP/mg enzyme min under the optimal conditions. Verification experiment (6.21 mu mol p-NP/mg enzyme min) confirmed the validity of the predicted model. Immobilized lipase was used to determine operational stability and it indicated that immobilized lipase was stable retaining more than 80% residual activity after being used repeatedly for 10 consecutive batches of pomace oil transesterification. (C) 2011 Elsevier Ltd. All right sreserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据