AtCYP710A1 gene-mediated stigmasterol production plays a role in imparting temperature stress tolerance in Arabidopsis thaliana

Stigmasterol and sitosterol, important sterols present in plants, are known to influence permeability and fluidity characteristics of the plasma membrane and other organellar membranes. We had previously demonstrated that the Arabidopsis Atcyp710A1 gene, which catalyzes conversion of sitosterol into stigmasterol, plays a role in plasma membrane permeability, thus influencing leakage of cellular nutrients and ions into apoplast. In this study, we investigated the role of this gene in imparting various abiotic stress tolerances in Arabidopsis. By analyzing Atcyp710a1 mutant and AtCYP710A1 overexpressor lines, we found that the AtCYP710A1 gene plays a role in imparting low and high temperature tolerance.