期刊
BMC MOLECULAR BIOLOGY
卷 11, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2199-11-14
关键词
-
资金
- Polish Ministry of Scientific Research and Information Technology [2P04A07829, 63/6PR-UE/2007/7]
- Wellcome Trust
- European Community [LSHM-CT-2006-036903]
Background: MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results: Here we report that the proinflammatory cytokine IL-1 beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1 beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions: We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1 beta stimulation.
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