期刊
ANALYTICAL CHEMISTRY
卷 87, 期 15, 页码 7865-7871出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b01570
关键词
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资金
- National Research Foundation (NRF) [2013R1A2A2A05004314, 2008-0061983, NRF-2014K2A2A2000944]
- Korean Government [IBS-R020-D1]
We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc. The presented organic solvent-tolerable (for n-hexane, ethanol) microfluidic disc was newly fabricated by combining thermal fusion bonding and carbon dot-based valving techniques. It is expected that this novel platform will possibly contribute toward sustainable biofuel applications by providing a practical solution for on-site monitoring of lipid accumulation in microalgal samples, thus providing imperative contribution toward energy and environmental purposes of centrifugal microfluidic technology.
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