期刊
BMC MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2180-13-20
关键词
Francisella tularensis; Type VI secretion; Cytopathogenicity; Intracellular replication; PdpC
类别
资金
- Swedish Research Council [2009-5026]
- Medical Faculty, Umea University, Umea, Sweden
Background: A prerequisite for the virulence of the facultative intracellular bacterium Francisella tularensis is effective intramacrophage proliferation, which is preceded by phagosomal escape into the cytosol, and ultimately leads to host cell death. Many components essential for the intracellular life cycle are encoded by a gene cluster, the Francisella pathogenicity island (FPI), constituting a type VI secretion system. Results: We characterized the FPI mutant Delta pdpC of the live vaccine strain (LVS) of F. tularensis and found that it exhibited lack of intracellular replication, incomplete phagosomal escape, and marked attenuation in the mouse model, however, unlike a phagosomally contained FPI mutant, it triggered secretion of IL-1 beta, albeit lower than LVS, and markedly induced LDH release. Conclusions: The phenotype of the Delta pdpC mutant appears to be unique compared to previously described F. tularensis FPI mutants.
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