期刊
BMC MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2180-12-188
关键词
Hrc1Type III secretion system; Pseudomonas syringae; Rhizobium Type III secretion system; Phylogenetic analysis; Pathogenicity; Gene organization; Horizontal transfer events; Common ancestry; Evolutionary relationships; RT-PCR
类别
资金
- PENED grant from the Greek Ministry of Education, GSRT
- PYTHAGORAS grant from the Greek Ministry of Education, GSRT
- PEP grant from the Greek Ministry of Education, GSRT [KP-15]
- EPEAEK-Plant Molecular Biology and Biotechnology
- Protein Biotechnology graduate programs
- Onassis Foundation fellowship
- GSRT post-doctoral grant
- Marie Curie Reintegration Grant
Background: The central role of Type III secretion systems (T3SS) in bacteria-plant interactions is well established, yet unexpected findings are being uncovered through bacterial genome sequencing. Some Pseudomonas syringae strains possess an uncharacterized cluster of genes encoding putative components of a second T3SS (T3SS-2) in addition to the well characterized Hrc1 T3SS which is associated with disease lesions in host plants and with the triggering of hypersensitive response in non-host plants. The aim of this study is to perform an in silico analysis of T3SS-2, and to compare it with other known T3SSs. Results: Based on phylogenetic analysis and gene organization comparisons, the T3SS-2 cluster of the P. syringae pv. phaseolicola strain is grouped with a second T3SS found in the pNGR234b plasmid of Rhizobium sp. These additional T3SS gene clusters define a subgroup within the Rhizobium T3SS family. Although, T3SS-2 is not distributed as widely as the Hrc1 T3SS in P. syringae strains, it was found to be constitutively expressed in P. syringae pv phaseolicola through RT-PCR experiments. Conclusions: The relatedness of the P. syringae T3SS-2 to a second T3SS from the pNGR234b plasmid of Rhizobium sp., member of subgroup II of the rhizobial T3SS family, indicates common ancestry and/or possible horizontal transfer events between these species. Functional analysis and genome sequencing of more rhizobia and P. syringae pathovars may shed light into why these bacteria maintain a second T3SS gene cluster in their genome.
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