期刊
FUTURE CARDIOLOGY
卷 9, 期 1, 页码 137-147出版社
FUTURE MEDICINE LTD
DOI: 10.2217/FCA.12.76
关键词
biomarker; impedance biosensor; impedance spectroscopy; introduction; nanoporous membrane; NT-proBNP
资金
- Office of Naval Research [N00014-07-1-0457]
Aim: To demonstrate a label-free electrical immunoassay for profiling vascular biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP) associated with improved cardiac risk prediction. Materials & methods: A high-density nanowellbased electrical immunoassay has been designed by integrating nanoporous aluminum oxide onto printed circuit board chips for the detection of NT-proBNP. The concentration of the biomarker is quantitatively determined by measuring impedance changes to the electrical double layer within the nanowells using electrochemical impedance spectroscopy. Detection sensitivity in the fg/ml range was obtained due to spatial confinement of the target biomarkers in size-matched nanowells. Results & discussion: Electrical immunoassay performance was determined for the detection of NT-proBNP in phosphate-buffered saline (PBS) and human serum (HS). The lower limit of detection for the sensor was observed to be 10 fg/ml in PBS and 500 fg/ml in HS. The upper limit of detection was observed to be 500 fg/ml in PBS and 500 ng/ml in HS. Conclusion: A label-free technique for detection of NT-proBNP at clinically relevant concentrations for evaluating cardiac risk is demonstrated. High sensitivity and specificity, robust detection and low volume (100 mu l) per assay project the technology to be a successful competitor to traditional ELISA-based
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