期刊
CLINICAL AND INVESTIGATIVE MEDICINE
卷 36, 期 2, 页码 E103-E111出版社
CANADIAN SOC CLINICAL INVESTIGATION
DOI: 10.25011/cim.v36i2.19573
关键词
-
资金
- National Natural Science Foundation of China [81072413, 31270973, 31170878, 31270977]
- Major State Basic Research Development Program of China [2013CB530501]
- Jiangsu Pan-Deng Project [BK2010004]
- natural science foundation of the Jiangsu higher education institutions [12KJB310015]
- Ph.D. Programs Foundation of Ministry of Education of China [20113201120011]
- Jiangsu 333 project of cultivation of high-level talents
- Qing Lan Project of the Jiangsu higher education institutions
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT-IRT1075]
Purpose: The participation of microRNAs (miRNAs) in cardiovascular diseases suggests them as potential targets for novel preventive and therapeutic strategies. In this study, the key myocardial miRNA, miR-21, was identified in the murine coxsackievirus B3 (CVB3)-induced myocarditis model and its contribution to disease progression was explored. Methods: Myocardial microRNA expression changes in CVB3-infected mice were analyzed by real-time PCR and miR-21 was found to be the miRNA whose expression was significantly reduced. Mice were injected with plasmid encoding miR-21 (pMDH-miR-21) at day 1 post CVB3 infection and myocarditis severity was evaluated 7 days post-infection. The underlying mechanism of miR-21 in viral myocarditis was also investigated. Results: Myocardial miR-21 expression was negatively related to viral myocarditis severity. Recovery of miR-21 expression, by injecting with pMDH-miR-21, significantly relieved CVB3-induced myocarditis as shown by increased body weight, reduced myocardial injury, lowered myocarditis score and increased survival rate. Further study showed that miR-21 could protect myocardial apoptosis by specifically inhibiting its target programmed cell death 4 (PDCD4) expression. Conclusion: miR-21 administration efficiently alleviated CVB3-induced myocarditis by repressing PDCD4-mediated apoptosis. Our study not only helps to better understand the pathogenesis of viral myocarditis, but also proves the potential of miR-21 as a novel therapeutic target for treatment of CVB3-induced myocarditis and other apoptosis-mediated cardiovascular diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据