期刊
BMC GENOMICS
卷 15, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2164-15-527
关键词
Drought; Recovery; Switchgrass; Panicum virgatum; Gene expression; RNA-seq
资金
- National Science Foundation Plant Genome Research Program Award [IOS-0922457]
- United States Department of Agriculture NIFA-AFRI postdoctoral fellowship [2011-67012-30696]
- Hawkesbury Institute for the Environment, University of Western Sydney
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0922457] Funding Source: National Science Foundation
- NIFA [2011-67012-30696, 687448] Funding Source: Federal RePORTER
Background: In light of the changes in precipitation and soil water availability expected with climate change, understanding the mechanisms underlying plant responses to water deficit is essential. Toward that end we have conducted an integrative analysis of responses to drought stress in the perennial C-4 grass and biofuel crop, Panicum virgatum (switchgrass). Responses to soil drying and re-watering were measured at transcriptional, physiological, and metabolomic levels. To assess the interaction of soil moisture with diel light: dark cycles, we profiled gene expression in drought and control treatments under pre-dawn and mid-day conditions. Results: Soil drying resulted in reduced leaf water potential, gas exchange, and chlorophyll fluorescence along with differential expression of a large fraction of the transcriptome (37%). Many transcripts responded differently depending on time of day (e.g. up-regulation pre-dawn and down-regulation mid-day). Genes associated with C-4 photosynthesis were down-regulated during drought, while C-4 metabolic intermediates accumulated. Rapid changes in gene expression were observed during recovery from drought, along with increased water use efficiency and chlorophyll fluorescence. Conclusions: Our findings demonstrate that drought responsive gene expression depends strongly on time of day and that gene expression is extensively modified during the first few hours of drought recovery. Analysis of covariation in gene expression, metabolite abundance, and physiology among plants revealed non-linear relationships that suggest critical thresholds in drought stress responses. Future studies may benefit from evaluating these thresholds among diverse accessions of switchgrass and other C-4 grasses.
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