期刊
BMC GENOMICS
卷 15, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2164-15-584
关键词
Desulfococcus biacutus; Acetone activation; Genome sequencing; Carbonylation; Thiamine diphosphate
资金
- GATC Biotech AG
- Andreas Marquardt
- Deutsche Forschungsgemeinschaft (DFG) in its SPP 1319 priority program [SCHL1936/1]
- Konstanz Research School Chemical Biology (KoRS-CB)
- University of Konstanz
- Konstanz Young Scholar Fund (YSF)
- DFG [SCHL1936/1]
Background: The sulfate-reducing bacterium Desulfococcus biacutus is able to utilize acetone for growth by an inducible degradation pathway that involves a novel activation reaction for acetone with CO as a co-substrate. The mechanism, enzyme(s) and gene(s) involved in this acetone activation reaction are of great interest because they represent a novel and yet undefined type of activation reaction under strictly anoxic conditions. Results: In this study, a draft genome sequence of D. biacutus was established. Sequencing, assembly and annotation resulted in 159 contigs with 5,242,029 base pairs and 4773 predicted genes; 4708 were predicted protein-encoding genes, and 3520 of these had a functional prediction. Proteins and genes were identified that are specifically induced during growth with acetone. A thiamine diphosphate-requiring enzyme appeared to be highly induced during growth with acetone and is probably involved in the activation reaction. Moreover, a coenzyme B-12-dependent enzyme and proteins that are involved in redox reactions were also induced during growth with acetone. Conclusions: We present for the first time the genome of a sulfate reducer that is able to grow with acetone. The genome information of this organism represents an important tool for the elucidation of a novel reaction mechanism that is employed by a sulfate reducer in acetone activation.
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