期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 53, 期 8, 页码 2716-2719出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00662-15
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资金
- Commission for Scientific Research in Greenland [0605-00062B]
- Seed Funding Initiative for Collaboration of the University of Southern Denmark
- Novo Nordisk Foundation [7651]
- European Union TB-PAN-NET [FP7-223681]
- Patho-Ngen-Trace projects [FP7-278864-2]
- German Center for Infection Research (DZIF)
- Seed Funding Initiative for Collaboration of the University of Kiel
- Seed Funding Initiative for Collaboration of the University of Hamburg
- Seed Funding Initiative for Collaboration of the University of Aarhus
- Lundbeck Foundation [R151-2013-14628]
- Lundbeck Foundation [R151-2013-14628] Funding Source: researchfish
- Novo Nordisk Fonden [NNF13OC0007651] Funding Source: researchfish
Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.
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