期刊
ANALYTICAL CHEMISTRY
卷 87, 期 3, 页码 1919-1924出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac504175h
关键词
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资金
- Pathology Department of Xiangya Hospital
- National Natural Science Foundation of China [NSFC 81370983, NSFC 21221003]
- Foundation of National Key Scientific Instrument and Equipment Development Projects [2011YQ0301241403]
- Hunan Province Natural Science Key Fund Project [2014SK2003]
- Foundation of China Hunan Provincial Science & Technology Department [2012FJ4371, S2014S2032]
- Special Fund for Young Teachers of Central South University [2012QNZT100]
- National Key Scientific Program of China [2011CB911000]
- U.S. National Institutes of Health [GM079359, CA133086]
Tissue immunostaining is critically important in clinical applications, and antibodies have been used extensively as the molecular probes. Recently, aptamer, as a new class of probes, have attracted much attention for their potential clinical and research value. Epithelial cell adhesion molecule (EpCAM) is a specific biomarker which is overexpressed in many cancers of epithelial origin. Here, a DNA-based EpCAM aptamer SYL3C is reported as a probe for the immunostaining of frozen and paraffin-embedded sections of colorectal cancer tissues. Commercialized EpCAM antibodies were also used as a standard control. EpCAM aptamer SYL3C specifically recognized and immunostained cancer nests of colorectal tumor sections, but it neither reacted with background cells within tumor sites nor exhibited cross-reaction to the benign lesions or inflammation of colorectal tissues. No cross-linking to EpCAM-negative malignant tumor sections occurred. Compared with standard antibody staining, our EpCAM aptamer SYL3C protocol is simpler to implement with a shorter reaction time. Moreover, SYL3C can specifically bind with either frozen or paraffin-embedded tissue sections. Since the histopathology of frozen tissue is closer to that of fresh tissue and since frozen sections can be produced more quickly than paraffin-embedded sections, SYL3C immunostaining of frozen sections is a quick protocol that is easy to implement.
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