期刊
BMC GENOMICS
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2164-12-612
关键词
-
资金
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) [N. 019/2006-CAPES/FAPEAM]
Background: Little is known about the composition and function of the saliva in black flies such as Simulium guianense, the main vector of river blindness disease in Brazil. The complex salivary potion of hematophagous arthropods counteracts their host's hemostasis, inflammation, and immunity. Results: Transcriptome analysis revealed ubiquitous salivary protein families-such as the Antigen-5, Yellow, Kunitz domain, and serine proteases-in the S. guianense sialotranscriptome. Insect-specific families were also found. About 63.4% of all secreted products revealed protein families found only in Simulium. Additionally, we found a novel peptide similar to kunitoxin with a structure distantly related to serine protease inhibitors. This study revealed a relative increase of transcripts of the SVEP protein family when compared with Simulium vittatum and S. nigrimanum sialotranscriptomes. We were able to extract coding sequences from 164 proteins associated with blood and sugar feeding, the majority of which were confirmed by proteome analysis. Conclusions: Our results contribute to understanding the role of Simulium saliva in transmission of Onchocerca volvulus and evolution of salivary proteins in black flies. It also consists of a platform for mining novel anti-hemostatic compounds, vaccine candidates against filariasis, and immuno-epidemiologic markers of vector exposure.
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