期刊
PLANT SIGNALING & BEHAVIOR
卷 9, 期 7, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/psb.29057
关键词
malate valve; plastidial glycolysis; ammonium assimilation; nitrate assimilation; energy supply; redox-balance
资金
- Deutsche Forschungsgemeinschaft [SFB 944]
- government of Lower Saxonia (Lichtenberg fellowship)
- Frauenfoerderpool of the University of Osnabrueck (fellowship)
In this study we analyzed the relationship between malate valve capacities, N-assimilation, and energy metabolism. We used transgenic plants either lacking the chloroplast NADP-dependent malate dehydrogenase or mutants with a decreased transcript level of the plastid-localized NAD-dependent malate dehydrogenase. Plants were grown on nitrate or ammonium, respectively, as the sole N-source and transcripts were analyzed by qRT -PCR. We could show that the lack of malate valve capacities enhances N-assimilation and plastidial glycolysis by increasing transcript levels of Fd-GOGAT s or NADH-GOGAT and plastidic NAD-GAPDHs (GapCps), respectively. Based on our results, we conclude that the lack of malate valve capacities is balanced by an increase of the activity of plastid-localized glycolysis in order to cover the high demand for plastidial ATP, stressing the importance of the plastids for energy metabolism in plant cells.
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