期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 54, 期 1, 页码 75-82出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02177-15
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资金
- Labex Integrative Biology of Emerging Infectious Diseases [ANR-10-LABX-62-IBEID]
- University Paris Descartes [641 113 10]
- Fondation pour la Recherche Medicale (FRM) [DEQ20100318279, DEQ20130326538]
- Laboratoire d'Excellence (LABEX) Integrative Biology of Emerging Infectious Diseases [ANR-10-LABX-62-IBEID]
- INSERM
- CNRS
- Universite Paris Descartes
- Institut Pasteur
- Institut de Veille Sanitaire
- Ministere de la Recherche et de l'Enseignement Superieur University Paris Descartes [641 113 10]
- Fondation pour la Recherche Medicale [DEQ20100318279, DEQ20130326538]
Group B Streptococcus (GBS) is a common commensal bacterium in adults, but is also the leading cause of invasive bacterial infections in neonates in developed countries. The beta-hemolysin/cytolysin (beta-h/c), which is always associated with the production of an orange-to-red pigment, is a major virulence factor that is also used for GBS diagnosis. A collection of 1,776 independent clinical GBS strains isolated in France between 2006 and 2013 was evaluated on specific medium for beta-h/c activity and pigment production. The genomic sequences of nonhemolytic and nonpigmented (NH/NP) strains were analyzed to identify the molecular basis of this phenotype. Gene deletions or complementations were carried out to confirm the genotype-phenotype association. Sixty-three GBS strains (3.5%) were NH/NP, and 47 of these (74.6%) originated from invasive infections, including bacteremia and meningitis, in neonates or adults. The mutations are localized predominantly in the cyl operon, encoding the beta-h/c pigment biosynthetic pathway and, in the abx1 gene, encoding a CovSR regulator partner. In conclusion, although usually associated with GBS virulence, beta-h/c pigment production is not absolutely required to cause human invasive infections. Caution should therefore be taken in the use of hemolysis and pigmentation as criteria for GBS diagnosis in routine clinical laboratory settings.
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