期刊
BMC GENETICS
卷 13, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1471-2156-13-98
关键词
Antimicrobial peptide; beta-defensins; Pigs; pBD; Pig genome; SNP
资金
- Cooperative Research Program for Agriculture Science & Technology Development Rural Development Administration, Republic of Korea [PJ009103]
- Technology Development Program for Bio-industry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea
Background: Beta-defensins (beta-defensins) are innate immune peptides with evolutionary conservation across a wide range of species and has been suggested to play important roles in innate immune reactions against pathogens. However, the complete beta-defensin repertoire in the pig has not been fully addressed. Result: A BLAST analysis was performed against the available pig genomic sequence in the NCBI database to identify beta-defensin-related sequences using previously reported beta-defensin sequences of pigs, humans, and cattle. The porcine beta-defensin gene clusters were mapped to chromosomes 7, 14, 15 and 17. The gene expression analysis of 17 newly annotated porcine beta-defensin genes across 15 tissues using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed differences in their tissue distribution, with the kidney and testis having the largest pBD expression repertoire. We also analyzed single nucleotide polymorphisms (SNPs) in the mature peptide region of pBD genes from 35 pigs of 7 breeds. We found 8 cSNPs in 7 pBDs. Conclusion: We identified 29 porcine beta-defensin (pBD) gene-like sequences, including 17 unreported pBDs in the porcine genome. Comparative analysis of beta-defensin genes in the pig genome with those in human and cattle genomes showed structural conservation of beta-defensin syntenic regions among these species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据