期刊
JOURNAL OF CLINICAL LABORATORY ANALYSIS
卷 30, 期 5, 页码 464-470出版社
WILEY
DOI: 10.1002/jcla.21880
关键词
clinical performance; Helicobacter pylori; limit of detection; loop-mediated isothermal amplification; sensitivity; stool antigen test
资金
- Iran National Science Foundation (INSF) [91002272]
Background: Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable. Methods: In this study, a loopmediated isothermal amplification (LAMP) targeted ureC of H. pylori was evaluated on 100 stool specimens and compared with a stool antigen test. Culture and rapid urease test were considered as gold standards. Results: The overall detection rate of the fecal antigen test and LAMP was 58% and 82%, respectively. The analytical sensitivity of the fecal antigen test and LAMP was 500 and 10 H. pylori cells/g and 10 fg DNA/reaction, which is equal to six H. pylori genome. Conclusion: LAMP technique has been characterized by high sensitivity and low detection limit for the detection of H. pylori in stool specimen. Clinical diagnostic performance of LAMP was better than the stool antigen test. (C) 2015 Wiley Periodicals, Inc.
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