期刊
CLINICAL PROTEOMICS
卷 11, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1559-0275-11-16
关键词
Two dimensional electrophoresis; Advances; Basics; Review
资金
- JSPS (Japan Society for Promotion of Science) from Ministry of Education, Culture, Sports, Science and Technology of Japan [23790933]
Gel-based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional-polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with mass spectrometric identification or other immunological tests. Although 2-DE was first introduced more than three decades ago, several challenges and limitations to its utility still exist. This review discusses the principles of 2-DE as well as both recent methodological advances and new applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据