期刊
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1472-6882-14-270
关键词
S-allyl mercaptocysteine; Breast cancer; Apoptosis; Cell cycle arrest
资金
- National 985 Project
- Special Project Fund of Shandong Province Taishan Scholars - Pharmaceutical Distinguished Experts
Background: S-allyl mercaptocysteine (SAMC), a water-soluble component derived from garlic, has been found to exert multi-antitumor activities. This study was to investigate the responsible molecular mechanisms of SAMC in human breast cancer cell lines. Methods: Sulforhodamine B assay was used to determine cell viability, flow cytometry was applied for the analysis of cell cycle and cell apoptosis, the change of protein was detected by Western blot. Results: It was found that SAMC exhibited an effective cell growth inhibition of human breast cancer cell lines MCF-7 (ER positive) and MDA-MB-231 (ER negative) in a dose-and time-dependent manner by inducing cell cycle arrested in G0/G1 phase, the block of cell cycle was associated with the up-regulation of p53 and p21. Furthermore, the SAMC-mediated cell cycle arrest was accompanied with promotion of apoptosis, as indicated by the changes in the nuclear morphology and expressions of apoptosis-related proteins. SAMC clearly triggered the mitochondrial apoptotic pathway as indicated by activation of Bax, decreased expression of Bcl-2 and Bcl-X-L, and subsequent activation of caspase-9 and caspase-3. Conclusion: These results highlight the value of a continued investigation into the use of SAMC as a potential antitumor candidate for breast cancer.
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