期刊
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1472-6882-14-54
关键词
Biyeom-Tang; Allergic rhinitis (AR); Bone-marrow derived mast cells (BMMC); Passive cutaneous anaphylaxis (PCA); Degranulation; Prostaglandin D-2 (PGD(2)); Leukotriene C-4 (LTC4)
资金
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009023]
Background: Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models. Methods: The anti-allergic properties of EBT were evaluated by measuring beta-Hex release and the production of prostaglandin D-2 (PGD(2)) and leukotriene C-4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo. Results: EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 mu g/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 mu g/ml and 10.9 mu g/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced. Conclusions: Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.
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