期刊
BMC BIOTECHNOLOGY
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1472-6750-14-25
关键词
Putative aminotransferase; PAMT; VAMT; Transaminase; Vanillylamine 1; phenylethylamine; Acetophenone; Whole-cell biocatalysis; Capsaicinoids
资金
- Swedish Research Council FORMAS
- Royal Swedish Academy of Agriculture and Forestry
- BIOFLAVOUR, COST Action [FA0907]
Background: The conversion of vanillin to vanillylamine is a key step in the biosynthetic route towards capsaicinoids in pungent cultivars of Capsicum sp. The reaction has previously been annotated to be catalysed by PAMT (putative aminotransferase; [GenBank: AAC78480.1, Swiss-Prot: O82521]), however, the enzyme has previously not been biochemically characterised in vitro. Results: The biochemical activity of the transaminase was confirmed by direct measurement of the reaction with purified recombinant enzyme. The enzyme accepted pyruvate, and oxaloacetate but not 2-oxoglutarate as co-substrate, which is in accordance with other characterised transaminases from the plant kingdom. The enzyme was also able to convert (S)-1-phenylethylamine into acetophenone with high stereo-selectivity. Additionally, it was shown to be active at a broad pH range. Conclusions: We suggest PAMT to be renamed to VAMT (vanillin aminotransferase, abbreviation used in this study) as formation of vanillin from vanillylamine could be demonstrated. Furthermore, due to high stereoselectivity and activity at physiological pH, VAMT is a suitable candidate for biocatalytic transamination in a recombinant whole-cell system.
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