期刊
BMC BIOTECHNOLOGY
卷 11, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1472-6750-11-27
关键词
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资金
- Netherlands organization for scientific research (NWO) [825.08.023]
- EMBO [ALTF-7272008]
- BMBF [PTJ-BIO 0313801L]
- European Commission [222965, 241587]
- DFG [SPP1170, WI 1058/6-3]
Background: The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagged recombinant proteins as a major contaminant when using this expression system. This is likely due to a histidine-rich C-terminus in GroEL1. Results: In order to improve purification efficiency and yield of polyhistidine-tagged mycobacterial target proteins, we created a mutant version of GroEL1 by removing the coding sequence for the histidine-rich C-terminus, termed GroEL1 Delta C. GroEL1 Delta C, which is a functional protein, is no longer able to bind nickel affinity beads. Using a selection of challenging test proteins, we show that GroEL1 Delta C is no longer present in protein samples purified from the groEL1 Delta C expression strain and demonstrate the feasibility and advantages of purifying and characterising proteins produced using this strain. Conclusions: This novel Mycobacterium smegmatis expression strain allows efficient expression and purification of mycobacterial proteins while concomitantly removing the troublesome contaminant GroEL1 and consequently increasing the speed and efficiency of protein purification.
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