期刊
BMB REPORTS
卷 47, 期 11, 页码 643-648出版社
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2014.47.11.097
关键词
Androgen receptor; RanBPM; STC2; Testosterone; Yeast two-hybrid
资金
- National Research Foundation of Korea (NRF) - Korean government (MEST) [2012R1A1A2044506, NRF-2009-0070344]
- Gangneung-Wonju National University
- National Research Foundation of Korea [2012R1A1A2044506, 2009-0070344] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Stanniocalcin (STC), a glycoprotein hormone originally discovered in fish, has been implicated in calcium and phosphate homeostasis. While fishes and mammals possess two STC homologs (STC1 and STC2), the physiological roles of STC2 are largely unknown compared with those of STC1. In this study, we identified Ran-binding protein M (RanBPM) as a novel binding partner of STC2 using yeast two-hybrid screening. The interaction between STC2 and RanBPM was confirmed in mammalian cells by immunoprecipitation. STC2 enhanced the RanBPM-mediated transactivation of liganded androgen receptor (AR), but not thyroid receptor beta, glucocorticoid receptor, or estrogen receptor beta. We also found that AR interacted with RanBPM in both the absence and presence of testosterone (T). Furthermore, we discovered that STC2 recruits RanBPM/AR complex in T-dependent manner. Taken together, our findings suggest that STC2 is a novel RanBPM-interacting protein that promotes AR transactivation.
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