期刊
BMB REPORTS
卷 42, 期 1, 页码 28-34出版社
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2009.42.1.028
关键词
Brassica napus; Osmotic stress; Ornithine delta-aminotransferase; Proline dehydrogenase; Delta(1)-pyrroline-5-carboxylate synthecase
资金
- Ministry of Science and Technology of China [2006CB100100]
- Hundred Talent Program of Chinese Academy of Science
To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding Delta(1)-pyrroline-5carboxylate synthetase (BnP5CS), ornithine delta-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development. [BMB reports 2009; 42(1): 28-34]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据