期刊
EUROPEAN THYROID JOURNAL
卷 4, 期 4, 页码 213-221出版社
KARGER
DOI: 10.1159/000441355
关键词
MicroRNA; Thyroid; Adenoma; Cell cycle
资金
- Associazione Italiana per la Ricerca sul Cancro-AIRC [IG 11477]
- Ministero dell'Universita e della Ricerca Scientifica e Tecnologica-MIUR
- P.O.R. Campania FSE [B25B09000050007]
- CNR Epigenomics Flagship Project 'EPIGEN'
We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity. (C) 2015 European Thyroid Association Published by S. Karger AG, Basel
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