期刊
JOURNAL OF EXTRACELLULAR VESICLES
卷 4, 期 -, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.3402/jev.v4.26048
关键词
EV Array; exosomes; phenotyping; protein microarray
类别
资金
- Toyota Fonden
- Det Obelske Familiefond
- Aalborg Frivillige Bloddonorers Fond
Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jorgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
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