期刊
JOURNAL OF EXTRACELLULAR VESICLES
卷 4, 期 -, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.3402/jev.v4.26883
关键词
exosomes; extracellular vesicles; nano LC-MS/MS; OptiMEM; pre-spun; proteome
类别
资金
- EU IMI
- Agency for Science, Technology and Research (A*STAR), Singapore
- Postdoctoral MOBILITAS Fellowship of Estonian Science Foundation
- Estonian Research Council [PUT618]
- Rubicon Fellowship from the Netherlands Organisation for Scientific Research (NWO)
- Karolinska Institutet
- Swedish Medical Research Council (VR-Med)
- Swedish Society of Medical Research (SSMF)
- EuroNanoMedII
- Swedish Research Council (VR-NT)
- Swedish Cancer Society
- Parkinson"
- s UK [G-1109] Funding Source: researchfish
Extracellular vesicles (EVs) play a significant role in cell-cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum-free (OptiMEM) conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum-containing (pre-spun) conditions, the quantity of isolated EVs was greatly increased. Moreover, the expression levels of certain vesicular proteins (e.g. small GTPases, G-protein complexes, mRNA processing proteins and splicing factors), some of which were previously reported to be involved in EV biogenesis, were found to be differentially expressed in EVs under different culture conditions. These data, therefore, contribute to the understanding of how extracellular factors and intracellular molecular pathways affect the composition and release of EVs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据