期刊
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
卷 100, 期 10, 页码 E1308-E1318出版社
ENDOCRINE SOC
DOI: 10.1210/jc.2015-1800
关键词
-
资金
- Genzyme-Sanofi Fellowship in Nephrology grant
- National Institute for Health Research (NIHR) award
- NIHR Grant [14/49/147]
- British Heart Foundation
- British Heart Foundation [FS/12/8/29377] Funding Source: researchfish
Context: alpha-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of alpha-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system wide tissue expression of transmembrane alpha-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human alpha-Klotho expression in health. Results: The distribution of alpha-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive, and neuronal tissues, was first identified by immunohistochemistry. Kidney tissues showed strong alpha-Klotho expression, whereas liver did not reveal a detectable signal. These results were next confirmed by Western blotting of both whole tissues and primary cells. To validate our antibody-based results, alpha-Klotho-expressing tissues were subjected to parallel reaction monitoring mass spectrometry (data deposited at ProteomeXchange, PXD002775) identifying peptides specific for the full-length, transmembrane alpha-Klotho isoform. Conclusions: The data presented confirm alpha-Klotho expression in the kidney tubule and in the artery and provide evidence of alpha-Klotho expression across organ systems and cell types that has not previously been described in humans.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据