期刊
BLOOD CELLS MOLECULES AND DISEASES
卷 52, 期 1, 页码 59-67出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bcmd.2013.07.013
关键词
Umbilical cord blood; CD34(+); Engraftment; Hyperbaric oxygen
类别
资金
- Office of Scholarly, Academic & Research Mentoring (OSARM), Department of Internal Medicine, University of Kansas Medical Center
- OSARM at home institution
- Robert K. Dempski Cord Blood Research Fund
- NIH/NIGMS COBRE grant [P30 GM103326]
Delayed engraftment and graft failure represent major obstacles to successful umbilical cord blood (UCB) transplantation. Herein, we evaluated the use of hyperbaric oxygen (HBO) therapy as an intervention to improve human UCB stem/progenitor cell engraftment in an immune deficient mouse model. Six- to eight-week old NSG mice were sublethally irradiated 24 hours prior to CD34(+) UCB cell transplant. Irradiated mice were separated into a non-HBO group (where mice remained under normoxic conditions) and the HBO group (where mice received 2 hours of HBO therapy; 100% oxygen at 2.5 atmospheres absolute). Four hours after completing HBO therapy, both groups intravenously received CD34(+) UCB cells that were transduced with a lentivirus carrying luciferase gene and expanded for in vivo imaging. Mice were imaged and then sacrificed at one of 10 times up to 4.5 months post-transplant HBO treated mice demonstrated significantly improved bone marrow, peripheral blood, and spleen retention and subsequent engraftment In addition, HBO significantly improved peripheral, spleen and bone marrow engraftment of human myeloid and B-cell subsets. In vivo imaging demonstrated that HBO mice had significantly higher ventral and dorsal bioluminescence values. These studies suggest that HBO treatment of NSG mice prior to UCB CD34(+) cell infusion significantly improves engraftment. (C) 2013 Elsevier Inc. All rights reserved.
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