期刊
BLOOD
卷 122, 期 4, 页码 523-532出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2013-01-481135
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资金
- Netherlands Organization for Scientific Research and TopTalent
- National Roadmap for Large Scale Infrastructure (Mouse Clinic for Cancer and Aging)
- Netherlands Institute for Regenerative Medicine
The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B cells is substantially different. Contributions of individual clones to blood are dynamically changing; most of the clones either expand or decline with time. Finally, we demonstrate that the pool of old HSCs is composed of multiple small clones, whereas the young HSC pool is dominated by fewer, but larger, clones.
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