期刊
BLOOD
卷 119, 期 26, 页码 6296-6306出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2011-09-376475
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资金
- Wellcome Trust
- National Health Service Blood and Transplant
- Department of Health (United Kingdom)
- BBSRC [BB/J002704/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J002704/1] Funding Source: researchfish
- National Institute for Health Research [RP-PG-0310-1004] Funding Source: researchfish
The erythrocyte is one of the best characterized human cells. However, studies of the process whereby human reticulocytes mature to erythrocytes have been hampered by the difficulty of obtaining sufficient numbers of cells for analysis. In the present study, we describe an in vitro culture system producing milliliter quantities of functional mature human adult reticulocytes from peripheral blood CD34(+) cells. We show that the final stage of reticulocyte maturation occurs by a previously undescribed mechanism in which large glycophorin A-containing vesicles forming at the cytosolic face of the plasma membrane are internalized and fuse with autophagosomes before expulsion of the autophagosomal contents by exocytosis. Early reticulocyte maturation is characterized by the selective elimination of unwanted plasma membrane proteins (CD71, CD98, and beta 1 integrin) through the endosome-exosome pathway. In contrast, late maturation is characterized by the generation of large glycophorin A-decorated vesicles of autophagic origin. (Blood. 2012;119(26):6296-6306)
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