期刊
BLOOD
卷 120, 期 4, 页码 914-923出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2011-12-398156
关键词
-
类别
资金
- Genome Network Project
- Special Coordination Funds for Promoting Science and Technology
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [23500488, 23122503]
- Grants-in-Aid for Scientific Research [22590282, 23122503, 23500488, 24791634] Funding Source: KAKEN
The early blood vessels of the embryo and yolk sac in mammals develop by aggregation of de novo-forming angio-blasts into a primitive vascular plexus, which then undergoes a complex remodeling process. Angiogenesis is also important for disease progression in the adult. However, the precise molecular mechanism of vascular development remains unclear. It is therefore of great interest to determine which genes are specifically expressed in developing endothelial cells (ECs). Here, we used Flk1-deficient mouse embryos, which lack ECs, to perform a genome-wide survey for genes related to vascular development. We identified 184 genes that are highly enriched in developing ECs. The human orthologs of most of these genes were also expressed in HUVECs, and small interfering RNA knockdown experiments on 22 human orthologs showed that 6 of these genes play a role in tube formation by HUVECs. In addition, we created Arhgef15 knockout and RhoJ knockout mice by a gene-targeting method and found that Arhgef15 and RhoJ were important for neonatal retinal vascularization. Thus, the genes identified in our survey show high expression in ECs; further analysis of these genes should facilitate our understanding of the molecular mechanisms of vascular development in the mouse. (Blood. 2012;120(4):914-923)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据