期刊
ANALYTICAL BIOCHEMISTRY
卷 486, 期 -, 页码 51-53出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2015.06.031
关键词
Detergent extraction; Homologous recombination; Seamless DNA cloning; SLiCE
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI Grant [25650037]
- Grants-in-Aid for Scientific Research [25650037] Funding Source: KAKEN
Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA cloning without the use of restriction enzymes. Instead, SLiCE uses homologous recombination activities from Escherichia coil cell lysates. To date, SLiCE preparation has been performed using an expensive commercially available lytic reagent. To expand the utility of the SLiCE method, we evaluated different methods for SLiCE preparation that avoid using this reagent. Consequently, cell extracts prepared with buffers containing Triton X-100, which is a common and low-cost nonionic detergent, exhibited sufficient cloning activity for seamless gene incorporation into a vector. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据