期刊
BLOOD
卷 117, 期 2, 页码 678-687出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2010-08-301333
关键词
-
类别
资金
- Swedish Research Council [71X-14251]
- Medical Faculty at Lund University
- ALF
- Skane county council's Research and Development foundation, Sweden
The A4GALT locus encodes a glycosyltransferase that synthesizes the terminal Gal alpha 1-4Gal of the P-k (Gb3/CD77) glycosphingolipid, important in transfusion medicine, obstetrics, and pathogen susceptibility. Critical nucleotide changes in A4GALT not only abolish Pk formation but also another Gal alpha 1-4Gal-defined antigen, P1, which belongs to the only blood group system for which the responsible locus remains undefined. Since known A4GALT polymorphisms do not explain the P1-P-k+phenotype, P-2, we set out to elucidate the genetic basis of P-1/P-2. Despite marked differences (P-1 > P-2) in A4GALT transcript levels in blood, luciferase experiments showed no difference between P-1/P-2-related promoter sequences. Investigation of A4GALT mRNA in cultured human bone marrow cells revealed novel transcripts containing only the noncoding exon 1 and a sequence (here termed exon 2a) from intron 1. These 5'-capped transcripts include poly-A tails and 3 polymorphic sites, one of which was P-1/P-2-specific among > 200 donors and opens a short reading frame in P-2 alleles. We exploited these data to devise the first genotyping assays to predict P1 status. P-1/P-2 genotypes correlated with both transcript levels and P1/P-k expression on red cells. Thus, P-1 zygosity partially explains the well-known interindividual variation in P1 strength. Future investigations need to focus on regulatory mechanisms underlying P1 synthesis. (Blood. 2011;117(2):678-687)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据