期刊
BLOOD
卷 115, 期 12, 页码 2491-2499出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2009-07-231993
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- National Science Council, Taiwan [NSC 96-2320-B-002074-MY3, NSC 98-3112-B-002-032]
The cell-surface straight and branched repeats of N-acetyllactosamine (LacNAc) units, called poly-LacNAc chains, characterize the histo-blood group i and I antigens, respectively. The transition of straight to branched poly-LacNAc chain (i to I) is determined by the I locus, which expresses 3 IGnT transcripts, IGnTA, IGnTB, and IGnTC. Our previous investigation demonstrated that the i-to-I transition in erythroid differentiation is regulated by the transcription factor CCAAT/enhancer binding protein alpha (C/EBP alpha). In the present investigation, the K-562 cell line was used as a model to show that the i-to-I transition is determined by the phosphorylation status of the C/EBP alpha Ser-21 residue, with dephosphorylated C/EBP alpha Ser-21 stimulating the transcription of the IGnTC gene, consequently resulting in I branching. Results from studies using adult erythropoietic and granulopoietic progenitor cells agreed with those derived using the K-562 cell model, with lentiviral expression of C/EBP alpha in CD34(+) hematopoietic cells demonstrating that the dephosphorylated form of C/EBP alpha Ser-21 induced the expression of I antigen, granulocytic CD15, and also erythroid CD71 antigens. Taken together, these results demonstrate that the regulation of poly-LacNAc branching (I antigen) formation in erythropoiesis and granulopoiesis share a commonmechanism, with dephosphorylation of the Ser-21 residue on C/EBP alpha playing the critical role. (Blood. 2010;115:2491-2499)
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