期刊
BLOOD
卷 115, 期 15, 页码 3158-3161出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2009-11-254284
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资金
- Ministry of Education, Culture, Science, Sports, and Technology, Japan [B01, 17016089]
- Grants for Research on the Human Genome, Tissue Engineering Food Biotechnology
- Ministry of Health, Labor, and Welfare, Japan
- Core Research for Evolutional Science and Technology of Japan
- College Women's Association of Japan
- Foundation for Promotion of Cancer Research
- Morinaga Hoshikai [20591252]
- Grants-in-Aid for Scientific Research [21591256, 17016089] Funding Source: KAKEN
We investigated human leukocyte antigen (HLA) expression on leukemic cells derived from patients at diagnosis and relapse after hematopoietic stem cell transplantation (HSCT) using flow cytometry with locus-specific antibodies. Two of 3 patients who relapsed after HLA-haploidentical HSCT demonstrated loss of HLA alleles in leukemic cells at relapse; on the other hand, no loss of HLA alleles was seen in 6 patients who relapsed after HLA-identical HSCT. Single-nucleotide polymorphism array analyses of sorted leukemic cells further revealed the copy number-neutral loss of heterozygosity, namely, acquired uniparental disomy on the short arm of chromosome 6, resulting in the total loss of the mismatched HLA haplotype. These results suggest that the escape from immunosurveillance by the loss of mismatched HLA alleles may be a crucial mechanism of relapse after HLA-haploidentical HSCT. Accordingly, the status of mismatched HLA on relapsed leukemic cells should be checked before donor lymphocyte infusion. (Blood. 2010;115(15):3158-3161)
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