期刊
BLOOD
卷 113, 期 21, 页码 5121-5124出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2008-12-193003
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资金
- Medical Research Council
- Leukemia Research Fund, United Kingdom
- MRC [MC_U137970202, G0800784] Funding Source: UKRI
- Medical Research Council [MC_U137970202, G0800784B, G0800784] Funding Source: researchfish
The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. Recently, we identified the Runx1 + 23 enhancer that targets reporter gene expression to the first emerging HSCs of the mouse embryo when linked to the heterologous hsp68 promoter. Endogenous Runx1 is transcribed from 2 alternative promoters, P1 and P2. Here, we examined the in vivo cis-regulatory potential of these alternative promoters and asked whether they act with and contribute to the spatiotemporal specific expression of the Runx1 + 23 enhancer. Our results firmly establish that, in contrast to zebrafish runx1, mouse Runx1 promoter sequences do not confer any hematopoietic specificity in transgenic embryos. Yet, both mouse promoters act with the + 23 enhancer to drive reporter gene expression to sites of HSC emergence and colonization, in a + 23-specific pattern. (Blood. 2009; 113: 5121-5124)
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