期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 4, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2016.00116
关键词
heterogeneity; single cell; isolation; analysis; sequencing
资金
- National Basic Research Program of China [2013CB531103]
- National Natural Science Foundation of China [91339113, 81270202, 81601179]
- Natural Science Foundation of Jiangxi Province of China [20161BAB204166, 20161BAB205212]
- Shenzhen Basic Research Program [20140825105648]
- Wuhan Science and Technology Bureau grant [2014060202010125]
- Hubei 100 Talents
- Wuhan 3551 Talents Program
Individual cell heterogeneity within a population can be critical to its peculiar function and fate. Subpopulations studies with mixed mutants and wild types may not be as informative regarding which cell responds to which drugs or clinical treatments. Cell to cell differences in RNA transcripts and protein expression can be key to answering questions in cancer, neurobiology, stem cell biology, immunology, and developmental biology. Conventional cell based assays mainly analyze the average responses from a population of cells, without regarding individual cell phenotypes. To better understand the variations from cell to cell, scientists need to use single cell analyses to provide more detailed information for therapeutic decision making in precision medicine. In this review, we focus on the recent developments in single cell isolation and analysis, which include technologies, analyses and main applications. Here, we summarize the historical background, limitations, applications, and potential of single cell isolation technologies.
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