期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 4, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2016.00131
关键词
reticulocyte-derived exosomes; vaccine; malaria; spleen; PD-1 cells; effector memory T-cells; pD1-cells
资金
- CNPq, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico -Brasil
- Agencia de Gestio d'Ajtits Universitaris i de Recerca (AGAUR) from the Catalan Government [2014FI_B00649]
- Agencia de GestiO d'Ajuts Universitaris i de Recerca [2015 FI_B 00367]
- Ministerio de Economia y Competitividad, Spain [AP2012-2145]
- Ministerio Espanol de Economia y Competitividad [SAF2012-35133]
- European Community's Seventh Framework Programme
- Fundacion Ramon Areces
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00002014] Funding Source: researchfish
Reticulocyte-derived exosomes (rex) are 30-100 nm membrane vesicles of endocytic origin released during the maturation of reticulocytes to erythrocytes upon fusion of multivesicular bodies with the plasma membrane. Combination of CpG-ODN with rex obtained from BALB/c mice infected with the reticulocyte-prone non-lethal P. yoelii 17X malaria strain (rexPy), had been shown to induce survival and long lasting protection. Here, we show that splenectomized mice are not protected upon rexPy+CpG inmunizations and that protection is restored upon passive transfer of splenocytes obtained from animals immunized with rexPy+CpG. Notably, rexPy immunization of mice induced changes in PD1(-) memory T cells with effector phenotype. Proteomics analysis of rexPy confirmed their reticulocyte origin and demonstrated the presence of parasite antigens. Our studies thus prove, for what we believe is the first time, that rex from reticulocyte-prone malarial infections are associated with splenic long-lasting memory responses. To try extrapolating these data to human infections, in vitro experiments with spleen cells of human transplantation donors were performed. Plasma-derived exosomes from vivax malaria patients (exPv) were actively uptaken by human splenocytes and stimulated spleen cells leading to changes in T cell subsets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据