期刊
ANALYTICAL BIOCHEMISTRY
卷 480, 期 -, 页码 49-57出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2015.04.010
关键词
Diethyl phthalate (DEP); Gold nanoparticle (GNP); Real-time immuno-PCR (rt-IPCR); Foodstuff samples
资金
- National Natural Science Foundation of China [21177082, 21477074]
- Science and Technology Commission of Shanghai Municipality in China [09JC1407600]
- science and technology innovation special fund development projects of Shanghai Jiao Tong University
A specific polyclonal antibody targeting diethyl phthalate (DEP) with the higher antibody titer at 1:120,000 has been obtained, and an ultrasensitive and high-throughput direct competitive gold nanoparticles improved real-time immuno-PCR (GNP-rt-IPCR) technique has been developed for detecting DEP in foodstuff samples. Under optimal conditions, a rather low linearity is achieved within a range of 4 pg L-1 to 40 ng L-1, and the limit of detection (LOD) is 1.06 pg L-1. Otherwise, the GNP-rt-IPCR technique is highly selective, with low cross-reactivity values for DEP analogs (<5%). Finally, the concentrations of DEP in foodstuff samples by the GNP-rt-IPCR method range from 0.48 to 41.88 mu g kg(-1). Satisfactory recoveries (88.39-112.79%) and coefficient of variation values (8.38-12.77%) are obtained. The consistency between the results obtained from GNP-rt-IPCR and gas chromatography-mass spectrometry (GC-MS) is 98.3%, which further proves that GNP-rt-IPCR is an accurate, reliable, rapid, ultrasensitive, and high-throughput method for batch determination of trace amounts of DEP in foodstuff samples. (C) 2015 Elsevier Inc. All rights reserved.
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