期刊
FEMS MICROBIOLOGY REVIEWS
卷 40, 期 1, 页码 117-132出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsre/fuv036
关键词
microbiota; confounding factors; animal facility; animal models; microbiome
类别
资金
- European grants [FP6 [MRTN-CT-035624], FP7 [FP7-200767]]
- Belgian grants (Interuniversity Attraction Poles-IAP) [6/18, IAP 7/32]
- Flemish grants (Research Foundation Flanders (FWO) [G0226.09, G.0875.11, G.0973.11, G.0531.16, G.0A45.12, G.0172, G.0787.13, G0C3114]
- Methusalem grant [BOF09/01M00709]
- Ghent University
- Foundation against Cancer [2012-188]
- Flanders Institute for Biotechnology (VIB)
- FWO, IWT
- Brussels Region (Innoviris)
- KU Leven
- Rega institute
- FWO [1298213N]
- [FP7 [HEALTH-F4-2012-305312]]
Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent models represent an invaluable tool to study the host-microbiota interface. Progress in technical and computational tools to investigate the composition and function of the microbiome has opened a new era of research and we gradually begin to understand the parameters that influence variation of host-associated microbial communities. To isolate true effects from confounding factors, it is essential to include such parameters in model intervention studies. Also, explicit journal instructions to include essential information on animal experiments are mandatory. The purpose of this review is to summarize the factors that influence microbiota composition in mice and to provide guidelines to improve the reproducibility of animal experiments.Given the unmet need for standardizing the experimental work flow related to gut microbial research in animals, guidelines are required to isolate true effects from confounding factors.Given the unmet need for standardizing the experimental work flow related to gut microbial research in animals, guidelines are required to isolate true effects from confounding factors.
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