期刊
JOURNAL OF EXTRACELLULAR VESICLES
卷 5, 期 -, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.3402/jev.v5.32945
关键词
extracellular vesicles; isolation; characterization; purification; flow cytometry; proteomics; RNA analysis; single vesicle analysis
类别
资金
- NCI NIH HHS [P01 CA098912] Funding Source: Medline
- NHLBI NIH HHS [R01 HL124187] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [P01CA098912] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL124187] Funding Source: NIH RePORTER
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.
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