期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 313, 期 3, 页码 H667-H675出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00183.2017
关键词
hamster; microcirculation; arterioles; myogenic tone
资金
- National Heart, Lung, and Blood Institute [HL-32469, P01-HL-070687]
- American Heart Association Fellowship [0815778G]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL070687, R01HL032469] Funding Source: NIH RePORTER
Myogenic tone is an important feature of arterioles and resistance arteries, but the mechanisms responsible for this hallmark characteristic remain unclear. We used pharmacological inhibitors to compare the roles played by phospholipase C (PLC; 10 mu M U73122), inositol 1,4,5-trisphosphate receptors (IP(3)Rs; 100 mu M 2-aminoethoxydiphenylborane), protein kinase C (10 mu M bisindolylmaleimide I), angiotensin II type 1 receptors (1 mu M losartan), Rho kinase (10 nM - 30 mu M Y27632 or 300 nM H1152), stretch-activated ion channels (10 nM - 1 mu M Gd3+ or 5 mu M spider venom toxin GsMTx-4) and L-type voltage-gated Ca2+ channels (0.3-100 mu M diltiazem) in myogenic tone of cannulated, pressurized (80 cmH(2)O), second-order hamster cremaster or cheek pouch arterioles. Effective inhibition of either PLC or IP(3)Rs dilated cremaster arterioles, inhibited Ca-2+ waves, and reduced global Ca2+ levels. In contrast, cheek pouch arterioles did not display Ca2+ waves and inhibition of PLC or IP(3)Rs had no effect on myogenic tone or intracellular Ca2+ levels. Inhibition of Rho kinase dilated both cheek pouch and cremaster arterioles with equal efficacy and potency but also reduced intracellular Ca2+ signals in both arterioles. Similarly, inhibition of mechanosensitive ion channels with Gd2+ or GsMTx-4 produced comparable dilation in both arterioles. Inhibition of L-type Ca2+ channels with diltiazem was more effective in dilating cremaster (86 +/- 5% dilation, n = 4) than cheek pouch arterioles (54 +/- 4% dilation, n = 6, P < 0.05). Thus, there are substantial differences in the mechanisms underlying myogenic tone in hamster cremaster and cheek pouch arterioles. Regional heterogeneity in myogenic mechanisms could provide new targets for drug development to improve regional blood flow in a tissue-specific manner. NEW & NOTEWORTHY Regional heterogeneity in the mechanisms of pressure-induced myogenic tone implies that resistance vessels may be able to alter myogenic signaling pathways to adapt to their environment. A better understanding of the spectrum of myogenic mechanisms could provide new targets to treat diseases that affect resistance artery and arteriolar function.
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