期刊
BIRTH DEFECTS RESEARCH PART B-DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY
卷 101, 期 3, 页码 283-291出版社
WILEY
DOI: 10.1002/bdrb.21111
关键词
tripterygium glycosides; triptolide; ovary; granulosa cells; TGF-beta 1
资金
- Ministry of Science and Technology of China [2010CB945104, 2007CB948004]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01030401]
- Maternal-Fetal Medicine from Jiangsu Province Health Department of China
- Key Laboratory of Obstetric & Gynecologic Diseases, Ministry of Health
- Jiangsu Key Laboratory for Molecular Medicine [BM2007208]
- Key Laboratory, Department of Health, Jiangsu Province, China [XK200709]
This study was carried out to investigate the impact of tripterygium glycosides (TGs) on ovarian function of female rats in vitro and in vivo. In vitro studies showed that TG induced cells decrease at G1 phase and inhibited cell proliferation in rat granulosa cells. In vivo, female rats were intragastrically administered with TG at the dose of 60 mg/kg/day for consecutive 50 days. TG caused a prolonged estrous cycle, and a significant reduction in ovarian index, serum E-2 level, and numbers of secondary and antral follicles (p < 0.05) in these rats. A significant reduction of viable embryos was demonstrated in TG-treated female rats after mating (p < 0.01). Further, we observed observed the reduced expression level of TGF-beta 1 after TG treatment in vitro and in vivo. Moreover, the expression of Smad2 and AKT was also decreased after TG treatment. These results suggest that TG can impair ovarian function through Smads-mediated TGF-beta 1 signal pathway. (C) 2014 Wiley Periodicals, Inc.
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