期刊
BIOFABRICATION
卷 9, 期 1, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/aa5ba7
关键词
membrane; cell co-culture; porous thin film; cell-cell interaction; barrier model
资金
- NIGMS of the National Institutes of Health [R35GM119623]
- Gleason Family Foundation
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R35GM119623] Funding Source: NIH RePORTER
Typical in vitro barrier and co-culture models rely upon thick semi-permeable polymeric membranes that physically separate two compartments. Polymeric track-etched membranes, while permeable to small molecules, are far from physiological with respect to physical interactions with co-cultured cells and are not compatible with high-resolution imaging due to light scattering and autofluorescence. Here we report on an optically transparent ultrathin membrane with porosity exceeding 20%. We optimize deposition and annealing conditions to create a tensile and robust porous silicon dioxide membrane that is comparable in thickness to the vascular basement membrane (100-300 nm). We demonstrate that human umbilical vein endothelial cells (HUVECs) spread and proliferate on these membranes similarly to control substrates. Additionally, HUVECs are able to transfer cytoplasmic cargo to adipose-derived stem cells when they are co-cultured on opposite sides of the membrane, demonstrating its thickness supports physiologically relevant cellular interactions. Lastly, we confirm that these porous glass membranes are compatible with lift-off processes yielding membrane sheets with an active area of many square centimeters. Webelieve that these membranes will enable new in vitro barrier and co-culture models while offering dramatically improved visualization compared to conventional alternatives.
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